ABO, Blood Group A Antigen Antibody - CD BioSciences

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ABO, Blood Group A Antigen Antibody

ABO, Blood Group A Antigen Antibody

SPA-00130

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Target Information
Target Name ABO
Gene Abbr. ABO
Gene ID 28
Full Name ABO, alpha 1-3-N-acetylgalactosaminyltransferase and alpha 1-3-galactosyltransferase
Alias A3GALNT, A3GALT1, GTB, NAGAT
Product Details
Host Mouse
Clonality Monoclonal
Clone No. HE-14
Isotype IgM Kappa
Immunogen Mixture of erythrocytes of blood group A1 and glycoprotein fraction isolated from the saliva of secretors with blood group A.
Usage
Application IHC, IF
Dilutions Immunofluorescence (0.5-1.0 µg/mL)
Reactivity Human
Specificity This antibody recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1, 2, 3, 4, 5, 6) and Forssmann antigen. It is also reactive with the immuno-dominant A trisaccharide. Blood group antigen expression in human colon cancer was studied by means of two monoclonal antibodies of broad anti-A (HE-14) and anti-type 3 and type 4 chain-based A and H (HE-10) specificity. These antigens were proved to re-appear in tumors of the distal colon, the HE-10 antibody reacting more frequently (9 out of 12 samples) than HE-14 (5 out of 12 samples) and frequently with supra-nuclear staining of the cytoplasm probably in those places of the Golgi apparatus where carbohydrate antigens are synthesized. This staining pattern is characteristic of HE-10 in normal colonic mucosa as well. With HE-14, staining was often absent in less differentiated tumors, while HE-10 did react in such tumors. In some cases, these two antibodies gave different staining patterns in parallel sections from the same tissue sample, primarily at the cellular level. Three out of 12 cases showed blood group antigen expression in the mucosa of the distal colon adjacent to the tumor only when HE-10 Ab was used.
Storage & Handling
Storage Buffer 10 mM PBS with 0.05% BSA.
Preservative 0.05% Sodium Azide
Storage Temp. Store at 4 °C.

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