AATK Knockout Cell Line - CD BioSciences

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AATK Knockout Cell Line

AATK Knockout Cell Line

SPL-00020

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Description
1bp insertion
Target Information
Target Name AATK
Gene Abbr. AATK
Gene ID 9625
Full Name apoptosis associated tyrosine kinase
Alias AATYK, AATYK1, LMR1, LMTK1, PPP1R77
Species Human
Genomic Locus chr17:81131104
Transcript NM_001080395
WT Expression Level 1.12 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing)
Introduction The protein encoded by this gene contains a tyrosine kinase domain at the N-terminus and a proline-rich domain at the C-terminus. This gene is induced during apoptosis, and expression of this gene may be a necessary pre-requisite for the induction of growth arrest and/or apoptosis of myeloid precursor cells. This gene has been shown to produce neuronal differentiation in a neuroblastoma cell line. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2011].
Product Details
Cell Line Model HAP1
Genotype HAP1 cell line, edited by CRISPR/Cas to contain a 1bp insertion in a coding exon of AATK.
Description 1bp insertion
Parental Cell Line C631
Guide RNA Sequence GAGTGGCAGGACGTACACGT
PCR Primer Forward: CCTTCCTTGACCTGCGAGAAG
Reverse: CACTCCAGAGCTGAGTCTTCC
Handling Specifications
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish.
Culture Medium IMDM + 10% FCS
Growth Properties Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Freeze Medium IMDM + 20% FCS + 10% DMSO
Biosafety Level BSL-1
Disclaimer This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.

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