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14-3-3 β/α Antibody
SPA-00033
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | 14-3-3 |
| Gene Abbr. | YWHAB |
| Gene ID | 7529 |
| Full Name | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta |
| Alias | GW128, HEL-S-1, HS1, KCIP-1, YWHAA |
| Introduction | The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ, and η that have been identified in mammals. The initially described α and δ isoforms are confirmed to be phosphorylated forms of β and ζ, respectively. Through their amino-terminal α helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules. The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation independent interactions has been observed. 14-3-3 binding masks specific sequences of the target protein, and therefore, modulates target protein localization, phosphorylation state, stability, and molecular interactions. 14-3-3 proteins may also induce target protein conformational changes that modify target protein function. Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities. Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH. |
| Usage | |
|---|---|
| Application | WB |
| Dilutions | Western Blot (1:1000) |
| MW(KDa) | 28 |
| Reactivity | Rat, Human, Mouse, Bovine, Canine, Chicken, Primate, Xenopus, Zebrafish |
| Specificity | Specific for the ~29k 14-3-3 beta/alpha protein. Immunolabeling of the 14-3-3 beta/alpha protein band is completely blocked by pre-adsorption of the antibody with the C-terminal peptide that was used to generate the antibody. |
| Storage & Handling | |
|---|---|
| Storage Buffer | 10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA and 50% Glycerol. |
| Preservative | No Preservative |
| Storage Temp. | Store at -20 °C. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.